They are heavily used in many microbiologyrelated industries and fields to culture various microorganisms and are meant to be a more efficient method for detection and enumeration compared to conventional plating techniques. Remove the spreader and leave the 3m petrifilm staph express plate undisturbed for at least one minute to permit the gel. Products for business products for consumers about 3m. With flat side down, place 3m petrifilm spreader on top film over hydration diluent. Quality indicator testing 3m petrifilm plates dksh. Use it to determine total aerobic bacterial populations and to identify coliform levels, e. Apr 18, 2016 the new 3m petrifilm plate reader software version 4. With this one easy test, youll have confirmed results in 24 to 48 hours. Petrifilm large square spreader on the top film over the inoculum. Combined with the 3m petrifilm plate reader will provide consistently accurate counts in just 4 seconds. The 3m petrifilm plate reader also automates logging of 128 symbology bar code labels, and allows details related to the technician, food type, food lot and dilution schemes to be electronically stored rather than transcribed by hand.
Jun 18, 2018 3mpetrifilm lactic acid bacteria count plate video duration. The global food safety landscape is continuously changing. May 03, 2016 3m food safety announced today the availability of software enhancements to its 3m petrifilm plate reader, a popular peripheral device that serves as an automated alternative to the more timeconsuming task of manually counting and documenting colonies of bacteria on 3m petrifilm plates indicator tests. With the pipette perpendicular to the 3m petrifilm plate, place hydration diluent onto the center of the bottom film.
All of our products are unconditionally guaranteed. Fifty plates take up as much space as one petri dish. Enumeration of waterborne escherichia coli with petrifilm. Do not use plates that show orange or brown discoloration.
Rapid aerobic count plates, rapid yeast and mold count plates, rapid e. Enumeration of waterborne escherichia coli with petrifilm plates. Unlike traditional agar methods, 3m petrifilm plates are ready to use no prep required. The spreaders are reusable and do not contact plating surface or sample. Pdf 3m petrifilm staph express count plate method for. The 3m petrifilm plate reader provides consistent, automated reading and recording of results of 3m petrifilm plates aer. Proven as reliable as the drbc and dg18 spread plate methods as well as the acidified pda and ygc agar pour plate methods, 3m petrifilm rapid yeast and mold count plates also enhance productivity by eliminating the inoculation of multiple spread plates, the addition of antibiotics or tartaric acid, and the agar preparation process altogether.
The following images are examples of the ppr spreaderliquefier counting rules applied to petrifilm ac plates. Figure 2 shows a petrifilm ac plate without colonies. Thats why for a limited time were including our vocabulary audio training program for free when you download spreeder today. Petrifilm can be kept for 18 months if refrigerated. These results suggested that the 3m petrifilm plate method could replace the conventional methods in the analysis of microorganism contamination measurement in meat products.
Carefully peel open the petrifilm plate being careful not to touch the nutrient gel with your fingers. Clear acrylic material allows the technician to observe and control spread. For coliform petrifilmwith flat side down, place spreader on top film. Place the 3m petrifilm flat spreader with the flat side down on the center of the plate. Maximize productivity by increasing your laboratory efficiency with 3m petrifilm plates.
The third tool offered in the speed reading bundle is spreeder cx 2019, an ereader that uses a tactic called rsvp rapid serial visual. Use 3m petrifilm coliform count plates within one month after opening. With a pipet perpendicular to the petrifilm plate, place 1 ml of sample inoculum onto the center of the bottom film. To get speed reading training lessons and courses from our worldleading speed reading. Evaluation of the petrifilm aerobic count plate for enumeration of aerobic marine bacteria from seawater and caulerpa lentillifera.
Pdf 3m petrifilm staph express count plate method for the. Lift the top film of the 3m petrifilm staph express count plate and place the disk in the well of the plate. Gently apply pressure on the spreader to distribute the inoculum over a circular area. The 3m petrifilm staph express count plate method was compared with aoac official method 975. The 3m petrifilm flat spreader is compatible with the following 3m petrifilm plates. Evaluation of the petrifilm plate method for the enumeration. Place the petrifilm aerobic count plate on a flat surface. Data can also be easily exported to laboratory information management systems and other reporting tools. For the uberpowerful speed ereading apps, see spreeder cx. Spreader used to distribute the sample evenly for 3m petrifilm rapid yeast and mold, salmonella express, and staph express count plates. The 3m petrifilm plate reader provides consistent, automated reading and recording of results of 3m petrifilm plates aer showing 1 to 1 of 1 1 pages.
Pres sure ap plied to a plas tic spreader placed on the top film. Petrifilm aerobic count plates these are the plates to use in place of regular nutrient agar plates. Use petrifilm plates within one month after opening. Comparison of performance of rapid petrifilm test method and. Transparent acrylic material allows the technician to observe and control spread. Each slim pack offers consistent, uniform testing media. Count 0 it is easy to interpret the petrifilm ac plate. The petrifilm aerobic count plate is a reliable, ready made medium system for enumerating total aerobic bacteria populations 1,2,3,4. After individual 3m petrifilm plates are inoculated and incubated, they can be easily loaded into the 3m petrifilm. For use with petrifilm tm plates to seal the plastic film sheets. With ridge side down, place the spreader on the top film over the inoculum. The 3m petrifilm aerobic count ac plate is a readymade culture medium system that contains standard methods nutrients, a coldwatersoluble gelling agent and an indicator that facilitates colony enumeration. Evaluation of the petrifilm aerobic count plate for. The spreader is reusable and does not contact plating surface or sample.
Once the sample has been prepared, the top film is lifted from the 3m petrifilm plate to expose the inoculation. Wait at least 10 minutes to permit the gel to form. This 3m petrifilm spreader is an easytouse tool designed to quickly and evenly distribute inoculum within the 3m petrifilm plate. If for any reason you are not satisfied with any item, you may return it for a replacement, a refund, or credit. Designed for dispersing liquids over a plate surface. Find new freedom with time to focus on whats really important. Petrifilm was placed on a flat surface and 1 ml of sample of each dilution was placed at the. Kudaka j1, horii t, tamanaha k, itokazu k, nakamura m, taira k, nidaira m, okano s, kitahara a. Especially useful if conducting air sampling experiments or other air pollution studies. The 3m petrifilm plate reader provides consistent, automated reading and recording of results of 3m petrifilm plates aerobic, coliform, e. Count 16 figure 3 shows a petrifilm ac plate with a few bacterial colonies. It can also be used to enumerate coliforms plus potential pathogens in 2448 hours.
Petrifilm aerobic count plates contain standard methods nutrients, a cold water soluble gelling agent and a tetrazolium indicator dye which facilitates colony enumeration. Easytouse tools designed to quickly and evenly distribute inoculum within the 3m petrifilm plate. Change location united states english legalprivacydmcaaccessibility statement. The area of this petrifilm ac plate thats covered by spreadersliquefiers is 16. Petrifilm provides accurate results in three easy steps. The petrifilm plate method was simpler and less timeconsuming in sample preparation and, in procedures, faster than the conventional method. Design easily spreads inoculum over a uniform area. Since the inoculated area of a petrifilm ac plate is 20 cm2, the area of this petrifilm ac plate thats covered by spreadersliquefiers is 50%. The 3m petrifilm plate is an allinone plating system made by the food safety division of the 3m company. Press gently on the center of the spreader to distribute the inoculum over the circular area. The detection limit for aerobic bacteria by the petrifilm tm technique versus the conventional direct plating were 16x109 cfug and 5x108 cfug respectively while the detection limit of li by the petrifilm tm technique versus the conventional coliform plate count cpc were 22x106 cfug and 12x105 cfug. This compact, desktop unit automates the imaging, interpretation and data mapping of 3m petrifilm plate counts, raising your labs productivity, reducing. The new 3m petrifilm plate reader software version 4. Proven as reliable as the sma plate method, 3m petrifilm rapid aerobic count plates help agar users avoid costly retesting and delayed results due to interpretation issues caused by spreader colonies.70 431 206 401 764 1261 849 993 1371 9 435 1212 1129 973 1219 141 1430 641 543 205 1009 181 1316 1465 226 346 788 543 1434 316 1535 41 945 1145 1476 1273 1460 804 894 190 118 202 316 523 197 1217